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gst gene fusion vector pgex 6p 1  (GE Healthcare)


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    GE Healthcare gst gene fusion vector pgex 6p 1
    Gst Gene Fusion Vector Pgex 6p 1, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 97/100, based on 21429 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gst gene fusion vector pgex 6p 1/product/GE Healthcare
    Average 97 stars, based on 21429 article reviews
    gst gene fusion vector pgex 6p 1 - by Bioz Stars, 2026-02
    97/100 stars

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    gst gene fusion vector pgex 6p 1  (GE Healthcare)
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    Recombinant expression of SEO and identification of superantigenic activity of SEO. (A) SDS-PAGE analysis of purified SEO. Lane 1, supernatants of Escherichia coli encoding glutathione S-transferase <t>(GST)-SEO;</t> Lane2, purified GST-SEO fusion protein; Lane3, purified SEO. (B) Secretion of IFN-γ in mouse spleen cells in response to stimulation with 0.01 to 10 μg/mL of SEO. Positive control cells were stimulated with SEA. Negative control cells were stimulated with BSA.
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    Recombinant expression of SEO and identification of superantigenic activity of SEO. (A) SDS-PAGE analysis of purified SEO. Lane 1, supernatants of Escherichia coli encoding glutathione S-transferase <t>(GST)-SEO;</t> Lane2, purified GST-SEO fusion protein; Lane3, purified SEO. (B) Secretion of IFN-γ in mouse spleen cells in response to stimulation with 0.01 to 10 μg/mL of SEO. Positive control cells were stimulated with SEA. Negative control cells were stimulated with BSA.
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    Recombinant expression of SEO and identification of superantigenic activity of SEO. (A) SDS-PAGE analysis of purified SEO. Lane 1, supernatants of Escherichia coli encoding glutathione S-transferase (GST)-SEO; Lane2, purified GST-SEO fusion protein; Lane3, purified SEO. (B) Secretion of IFN-γ in mouse spleen cells in response to stimulation with 0.01 to 10 μg/mL of SEO. Positive control cells were stimulated with SEA. Negative control cells were stimulated with BSA.

    Journal: Frontiers in Immunology

    Article Title: ATP Facilitates Staphylococcal Enterotoxin O Induced Neutrophil IL-1β Secretion via NLRP3 Inflammasome Dependent Pathways

    doi: 10.3389/fimmu.2021.649235

    Figure Lengend Snippet: Recombinant expression of SEO and identification of superantigenic activity of SEO. (A) SDS-PAGE analysis of purified SEO. Lane 1, supernatants of Escherichia coli encoding glutathione S-transferase (GST)-SEO; Lane2, purified GST-SEO fusion protein; Lane3, purified SEO. (B) Secretion of IFN-γ in mouse spleen cells in response to stimulation with 0.01 to 10 μg/mL of SEO. Positive control cells were stimulated with SEA. Negative control cells were stimulated with BSA.

    Article Snippet: The pGEX-6P-1 glutathione S-transferase (GST) gene fusion vector was digested with Bam HI and Eco RI (Takara, Dalian, China).

    Techniques: Recombinant, Expressing, Activity Assay, SDS Page, Purification, Positive Control, Negative Control